Journal: Journal of Neurophysiology

Article Title: Visual responses in the dorsal lateral geniculate nucleus at early stages of retinal degeneration in rd 1 PDE6β mice

doi: 10.1152/jn.00231.2019

Figure Lengend Snippet: Spatial receptive fields in the rd1 Opn1mwR dorsal lateral geniculate nucleus (dLGN) A and B: effective photon flux (mean ± SE) of the background and bar stimuli used for receptive field mapping in the Opn1mwR and rd1 Opn1mwR mice, respectively, with calculated Michaelson contrast (%, mean ± SE) for each photopigment. Note that rod contrast is not relevant for rd1 mice because these animals lack rods at the age of recording. C: heat map for representative single units from the dLGN of an Opn1mwR (top) and rd1 Opn1mwR (bottom) mouse showing change in firing rate (spikes/s; scale at right) in response to the appearance of vertical bars (250 ms starting at time 0; 13° width at 4.5° resolution) as a function of location on azimuth of bar center. D: peak response amplitude (change in firing rate, mean ± SE) as a function of bar position for the 2 units in C, fit with a Gaussian function. E: box-and-whisker plot showing that receptive field diameter (mean ± SE) for all light-responsive units was significantly larger in Opn1mwR (12.17 ± 0.5°; n = 38 units; green bar) than in rd1 Opn1mwR mice (9.96 ± 0.3°; n = 48 units; black bar; ***P = 0.0005, unpaired t test). Box shows interquartile range; line in box is the median; cross is the mean; and whiskers indicate minimum to maximum range. F: peak response amplitude (change in firing rate, mean ± SE) was significantly larger in Opn1mwR (10.1 ± 1.2 spikes/s) than in rd1 Opn1mwR mice (7.02 ± 0.8 spikes/s; *P = 0.03, unpaired t test). G: response latency (mean ± SE) was significantly increased in rd1 Opn1mwR (177.9 ± 5.4 ms) compared with Opn1mwR mice (112.3 ± 4.46 ms; ****P < 0.0001, unpaired t test).

Article Snippet: The spatial receptive field size for single units meeting this criterion was estimated by fitting a two-dimensional Gaussian fit ( R 2 > 0.7) to the relationship between response amplitude and bar position in GraphPad Prism 7 (GraphPad Software).

Techniques: Whisker Assay

Journal: Scientific Reports

Article Title: Similar contrast sensitivity functions measured using psychophysics and optokinetic nystagmus

doi: 10.1038/srep34514

Figure Lengend Snippet: ( a ) The mean total distance moved by the eye of one observer that was consistent with OKN in the stimulus direction, for 16 drifting noise trials across five spatial frequencies, and eight velocities. Note substantially more OKN consistent motion at lower SFs. ( b ) As ( a ) but showing the total proportion of eye movements consistent with OKN in the stimulus direction (C θ ). ( c ) The optimum velocity (derived from log-Gaussian fits to 6 observers similar to ( b )) as a function of SF. ( d ) As for ( c ) but showing the amplitude of the log-Gaussian fit (a measure of signal-to-noise ratio) which does not show a strong dependence on SF, within observer.

Article Snippet: Patterns were two-dimensional Gaussian noise (made with the randn command in Matlab), filtered to be isotropic and spatial-frequency (SF) band-pass ( ).

Techniques: Derivative Assay